In Latin America, the agenda of sexual and reproductive rights advocated by the feminist and LGBTI movements has challenged the hegemony of the sexual order held by traditionalist sectors, especially the hierarchy of the Catholic Church and conservative evangelical churches. These religious groups have reacted, in turn, to arrest the advance of feminist and LGBTI agendas. Beyond conservative Catholic and evangelical hierarchies, opposition activists also include religious academic institutions, politicians, Christian lay movements, and civil society groups, among others, all committed to a more restrictive view of sexuality. One important strategy of this ‘Pro-Life’ activism in recent years has been the conformation of non governmental organizations (NGOs). This article offers an analysis of the emergence and development of ‘Pro-Life’ NGOs in Argentina. Using both quantitative and qualitative data, it examines three strategic movements made by these NGOs from the 1980s to the present: a state-political turn that favored strategies aimed to colonize the state and to impact sexual policies and the law; a blurring of religious identities; and a process of federalization and civil ecumenism.